Unzipping Kinetics of Double-Stranded DNA in a Nanopore

Details Unzipping Kinetics of Double-Stranded DNA in a Nanopore Unzipping Kinetics of Double-Stranded DNA in a Nanopore

2002-09-17

arxiv.org/abs/cond-mat/0209414v3

Physics

Condensed Matter

Soft Condensed Matter

10 pages, 5 figures, Accepted: Physics Review Letters

Scientific paper

10.1103/PhysRevLett.90.238101

We studied the unzipping kinetics of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. PCR analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip each molecule was inferred from the ionic current signature of DNA traversal. The distribution of times to unzip under various experimental conditions fit a simple kinetic model. Using this model, we estimated the enthalpy barriers to unzipping and the effective charge of a nucleotide in the pore, which was considerably smaller than previously assumed.

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