Probing the mechanical unzipping of DNA

Details Probing the mechanical unzipping of DNA Probing the mechanical unzipping of DNA

2005-12-20

arxiv.org/abs/cond-mat/0512487v2

Phys. Rev. Lett. 96, 248101 (2006)

Physics

Condensed Matter

Soft Condensed Matter

Scientific paper

10.1103/PhysRevLett.96.248101

A study of the micromechanical unzipping of DNA in the framework of the Peyrard-Bishop-Dauxois model is presented. We introduce a Monte Carlo technique that allows accurate determination of the dependence of the unzipping forces on unzipping speed and temperature. Our findings agree quantitatively with experimental results for homogeneous DNA, and for $\lambda$-phage DNA we reproduce the recently obtained experimental force-temperature phase diagram. Finally, we argue that there may be fundamental differences between {\em in vivo} and {\em in vitro} DNA unzipping.

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