Infra-Red Surface-Plasmon-Resonance technique for biological studies

Details Infra-Red Surface-Plasmon-Resonance technique for biological studies Infra-Red Surface-Plasmon-Resonance technique for biological studies

2007-11-18

arxiv.org/abs/0711.2786v1

Physics

Condensed Matter

Soft Condensed Matter

8 pages,8 figures

Scientific paper

10.1063/1.2828162

We report on a Surface-Plasmon-Resonance (SPR) technique based on Fourier -Transform - Infra - Red (FTIR) spectrometer. In contrast to the conventional surface plasmon technique, operating at a fixed wavelength and a variable angle of incidence, our setup allows the wavelength and the angle of incidence to be varied simultaneously. We explored the potential of the SPR technique in the infrared for biological studies involving aqueous solutions. Using computer simulations, we found the optimal combination of parameters (incident angle, wavelength) for performing this task. Our experiments with physiologically important glucose concentrations in water and in human plasma verified our computer simulations. Importantly, we demonstrated that the sensitivity of the SPR technique in the infrared range is not lower and in fact is even higher than that for visible light. We emphasize the advantages of infra red SPR for studying glucose and other biological molecules in living cells.

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