Physics – Condensed Matter – Materials Science
Scientific paper
2009-05-03
Nature 463, 214-217 (2010)
Physics
Condensed Matter
Materials Science
30 pages
Scientific paper
10.1038/nature08705
The ability to determine the structure of matter in three dimensions has profoundly advanced our understanding of nature. Traditionally, the most widely used schemes for 3D structure determination of an object are implemented by acquiring multiple measurements over various sample orientations, as in the case of crystallography and tomography (1,2), or by scanning a series of thin sections through the sample, as in confocal microscopy (3). Here we present a 3D imaging modality, termed ankylography (derived from the Greek words ankylos meaning 'curved' and graphein meaning 'writing'), which enables complete 3D structure determination from a single exposure using a monochromatic incident beam. We demonstrate that when the diffraction pattern of a finite object is sampled at a sufficiently fine scale on the Ewald sphere, the 3D structure of the object is determined by the 2D spherical pattern. We confirm the theoretical analysis by performing 3D numerical reconstructions of a sodium silicate glass structure at 2 Angstrom resolution and a single poliovirus at 2 - 3 nm resolution from 2D spherical diffraction patterns alone. Using diffraction data from a soft X-ray laser, we demonstrate that ankylography is experimentally feasible by obtaining a 3D image of a test object from a single 2D diffraction pattern. This approach of obtaining complete 3D structure information from a single view is anticipated to find broad applications in the physical and life sciences. As X-ray free electron lasers (X-FEL) and other coherent X-ray sources are under rapid development worldwide, ankylography potentially opens a door to determining the 3D structure of a biological specimen in a single pulse and allowing for time-resolved 3D structure determination of disordered materials.
Du Jincheng
Fahimian Benjamin P.
Jiang Huaidong
Kapteyn Henry C.
Miao Jianwei
No associations
LandOfFree
Three-dimensional structure determination from a single view does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Three-dimensional structure determination from a single view, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Three-dimensional structure determination from a single view will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-109254