Physics – Condensed Matter – Soft Condensed Matter
Scientific paper
2011-07-21
Poverkhnost' 2000, No7, pp. 88-91
Physics
Condensed Matter
Soft Condensed Matter
in Russian
Scientific paper
The process of compaction of high molecular weight DNA T4 is investigated directly in a AFM liquid cell. The AFM-images of globules formed by DNA molecules in the result of compaction in water-alcohol environments at high izopropanol concentration (80%) are received; it is found that at intermediate concentration of izopropanol (40-50%) the DNA molecules form partially compacted formations in which the separate coils of macromolecules twist in toroidal structures. It is shown using the technique of deconvolution of the AFM-images that the globule include only one closely packed DNA molecule. The model of DNA packing is proposed on the basis of AFM experiment.
Gallyamov Marat Olegovich
Pyshkina O. A.
Sergeyev V. G.
Yaminsky I. V.
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