Structural evolution of protein-biofilms: Simulations and Experiments

Details Structural evolution of protein-biofilms: Simulations and Experiments Structural evolution of protein-biofilms: Simulations and Experiments

2010-05-11

arxiv.org/abs/1005.1868v1

Physics

Condensed Matter

Soft Condensed Matter

13 pages, 10 figures; submitted

Scientific paper

The control of biofilm formation is a challenging goal that has not been reached yet in many aspects. One is the role of van der Waals forces and another the importance of mutual interactions between the adsorbing and the adsorbed biomolecules ('critical crowding'). Here, a combined exeperimental and theoretical approach is presented that fundamentally probes both aspects. On three model proteins, lysozyme, {\alpha}-amylase and bovine serum albumin (BSA), the adsorption kinetics is studied. Composite substrates are used enabling a separation of the short- and the long-range forces. Though usually neglected, experimental evidence is given for the influence of van der Waals forces on the protein adsorption as revealed by in situ ellipsometry. The three proteins were chosen for their different conformational stability in order to investigate the influence of conformational changes on the adsorption kinetics. Monte Carlo simulations are used to develop a model for these experimental results by assuming an internal degree of freedom to represent conformational changes. The simulations also provide data on the distribution of adsorption sites. By in situ atomic force microscopy we can also test this distribution experimentally which opens the possibility to e.g. investigate the interactions between adsorbed proteins.

Affiliated with

Also associated with

No associations

Rating

Structural evolution of protein-biofilms: Simulations and Experiments does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Structural evolution of protein-biofilms: Simulations and Experiments, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Structural evolution of protein-biofilms: Simulations and Experiments will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-385051