Physics – Condensed Matter – Soft Condensed Matter
Scientific paper
2003-07-04
Physics
Condensed Matter
Soft Condensed Matter
13 pages, 4 figures
Scientific paper
10.1063/1.1625420
We present results from an instrument combining surface acoustic wave (SAW) propagation and surface plasmon resonance (SPR) measurements. The objective is to use two independent methods, the former based on adsorbed mass change measurements and the latter on surface dielectric properties variations, to identify physical properties of protein layers, and more specifically their water content. We display mass sensitivity calibration curves using electrodeposition of copper leading to a sensitivity in liquid of 150$\pm15$ $cm^2/g$ for the Love mode device used here, and the application to monitoring biological processes. The extraction of protein layer thickness and protein to water content ratio is also presented for S-layer proteins under investigation. We obtain respectively 4.7$\pm$0.7 nm and 75$\pm$15%.
Campitelli Andrew
Francis Laurent
Friedt Jean-Michel
Palma R. de
Reekmans G.
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