Scanning probe microscopy of nucleic acids and thin organic films

Details Scanning probe microscopy of nucleic acids and thin organic films Scanning probe microscopy of nucleic acids and thin organic films

2011-04-24

arxiv.org/abs/1104.4632v1

Physics

Condensed Matter

Soft Condensed Matter

27 pages, in Russian

Scientific paper

We developed the models and algorithms to describe two main artefacts of AFM: (i) broadening effect and (ii) decreased heights of profiles for individual objects adsorbed on a hard substrate. It was shown how to measure elastic properties of a single adsorbed microobject. From the viewpoint of contact deformation theory we analysed mechanism of AFM visualisation of an atomic (molecular) structure of a flat surface. We tested technique of immobilisation on a substrate for free single-stranded RNA molecules in an extended state. Using AFM we visualised stages of processes of RNA release from protein coat of tobacco mosaic virus particles. The asymmetry of this process regarding two ends of a macromolecule was confirmed. The dynamics of compaction for DNA T4 molecules was traced using AFM in real time regime. The partially compacted macromolecules were clearly resolved. We detected that the partially compacted structures consisted of toroidal parts formed by different macromolecular strands. The real geometry of the compacted structures was reconstructed on the basis of systematic AFM measurements. That allowed us to calculate the amount of molecules combining each condensed DNA particle. We demonstrated clear benefits of horizontal deposition method for formation of LB films. Using AFM we achieved molecular resolution for some thin film coating and detected lattice parameters with the precision determined by the errors within a few percent. We demonstrated that the structure of the film is determined by the concurrence of several factors: by the closest packing principle for hydrocarbon tails, by the values of surface areas of polar heads at water subphase as well as by the substrate influence.

Affiliated with

Also associated with

No associations

Rating

Scanning probe microscopy of nucleic acids and thin organic films does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Scanning probe microscopy of nucleic acids and thin organic films, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Scanning probe microscopy of nucleic acids and thin organic films will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-624178