Probing the membrane potential of living cells by dielectric spectroscopy

Details Probing the membrane potential of living cells by dielectric spectroscopy Probing the membrane potential of living cells by dielectric spectroscopy

2008-12-17

arxiv.org/abs/0812.3352v1

Eur. Biophys. J (2009)

Physics

Condensed Matter

Soft Condensed Matter

Scientific paper

10.1007/s00249-009-0507-0

In this paper we demonstrate a quantitative way to measure the membrane potential of live cells by dielectric spectroscopy. We also show that the values of the membrane potential obtained using our technique are in good agreement with those obtained using traditional methods-voltage sensitive dyes. The membrane potential is determined by fitting the experimental dielectric dispersion curves with the dispersion curves obtain from a theoretical model. Variations in the membrane potential were induced by modifying the concentration of potassium chloride in the solution of the cell suspension in the presence of valinomycin. For exemplification of the method, E. coli were chosen for our experiments.

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