Membrane bound protein diffusion viewed by fluorescence recovery after bleaching experiments : models analysis

Details Membrane bound protein diffusion viewed by fluorescence recovery after bleaching experiments : models analysis Membrane bound protein diffusion viewed by fluorescence recovery after bleaching experiments : models analysis

2002-10-31

arxiv.org/abs/cond-mat/0210703v1

Physics

Condensed Matter

Statistical Mechanics

Scientific paper

Diffusion processes in biological membranes are of interest to understand the macromolecular organisation and function of several molecules. Fluorescence Recovery After Photobleaching (FRAP) has been widely used as a method to analyse this processes using classical Brownian diffusion model. In the first part of this work, the analytical expression of the fluorescence recovery as a function of time has been established for anomalous diffusion due to long waiting times. Then, experimental fluorescence recoveries recorded in living cells on a membrane-bound protein have been analysed using three different models : normal Brownian diffusion, Brownian diffusion with an immobile fraction and anomalous diffusion due to long waiting times.

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