Measurements of Protein-Protein Interactions by Size Exclusion Chromatography

Details Measurements of Protein-Protein Interactions by Size Exclusion Chromatography Measurements of Protein-Protein Interactions by Size Exclusion Chromatography

2003-06-12

arxiv.org/abs/cond-mat/0306346v1

Physics

Condensed Matter

Soft Condensed Matter

18 pages including 1 table and 5 figures

Scientific paper

10.1016/S0006-3495(03)74684-0

A method is presented for determining second virial coefficients B_2 of protein solutions from retention time measurements in size exclusion chromatography (SEC). We determine B_2 by analyzing the concentration dependance of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B_2 from positive to negative values in lysozyme and bovine serum albumin solutions. Our SEC results agree quantitatively with data obtained by light scattering.

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