Physics – Condensed Matter – Soft Condensed Matter
Scientific paper
2009-01-10
Rev. Sci. Instrum. 80, 014301 (2009)
Physics
Condensed Matter
Soft Condensed Matter
8 pages, 7 figures
Scientific paper
10.1063/1.3053242
We present an apparatus optimized for tracking swimming microorganisms in the size range 10-1000 microns, in three dimensions (3D), far from surfaces, and with negligible background convective fluid motion. CCD cameras attached to two long working distance microscopes synchronously image the sample from two perpendicular directions, with narrowband dark-field or bright-field illumination chosen to avoid triggering a phototactic response. The images from the two cameras can be combined to yield 3D tracks of the organism. Using additional, highly directional broad-spectrum illumination with millisecond timing control the phototactic trajectories in 3D of organisms ranging from Chlamydomonas to Volvox can be studied in detail. Surface-mediated hydrodynamic interactions can also be investigated without convective interference. Minimal modifications to the apparatus allow for studies of chemotaxis and other taxes.
Drescher Knut
Goldstein Raymond E.
Leptos Kyriacos C.
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