Physics
Scientific paper
Nov 2008
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2008natur.456..464l&link_type=abstract
Nature, Volume 456, Issue 7221, pp. 464-469 (2008).
Physics
9
Scientific paper
Protein-RNA interactions have critical roles in all aspects of gene expression. However, applying biochemical methods to understand such interactions in living tissues has been challenging. Here we develop a genome-wide means of mapping protein-RNA binding sites in vivo, by high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP). HITS-CLIP analysis of the neuron-specific splicing factor Nova revealed extremely reproducible RNA-binding maps in multiple mouse brains. These maps provide genome-wide in vivo biochemical footprints confirming the previous prediction that the position of Nova binding determines the outcome of alternative splicing; moreover, they are sufficiently powerful to predict Nova action de novo. HITS-CLIP revealed a large number of Nova-RNA interactions in 3' untranslated regions, leading to the discovery that Nova regulates alternative polyadenylation in the brain. HITS-CLIP, therefore, provides a robust, unbiased means to identify functional protein-RNA interactions in vivo.
Blume John E.
Chi Sung Wook
Clark Tyson A.
Darnell Jennifer C.
Darnell Robert B.
No associations
LandOfFree
HITS-CLIP yields genome-wide insights into brain alternative RNA processing does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with HITS-CLIP yields genome-wide insights into brain alternative RNA processing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and HITS-CLIP yields genome-wide insights into brain alternative RNA processing will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-1285077