Fractionation of sulfur isotopes by the yeast Saccharomyces cerevisiae

Physics

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Scientific paper

Growing and resting cell suspensions of Baker's yeast ( Saccharomyces cerevisiae ) were permitted to metabolize sulfite and sulfate under a variety of environmental conditions. Enrichment of S 32 in the H 2 S released during reduction of sulfate under growing conditions was consistently lower ( S 34 < -25%.) than enrichment during reduction of sulfite under similar conditions ( S 34 ~ -30 to -50%.). Yeast cells harvested from a sulfite medium released small quantities of H 2 S when suspended in glucose-sulfate solution; cells from a sulfate growth medium did not. The enrichment of H 2 S in S 32 by sulfite-grown resting cells suspended in either SO 2- 4 or SO 2- 3 solutions was about 10%.in less than the maximum obtained during growth on sulfite. Isotopic fractionation was always significantly less in assimilated, than in dissimilated sulfur.

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