Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase

Details Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase

2009-02-02

arxiv.org/abs/0902.0389v1

Biology

Quantitative Biology

Biomolecules

10 pages, 4 figures

Scientific paper

10.1117/12.812720

Color centers in diamond nanocrystals are a new class of fluorescence markers that attract significant interest due to matchless brightness, photostability and biochemical inertness. Fluorescing diamond nanocrystals containing defects can be used as markers replacing conventional organic dye molecules, quantum dots or autofluorescent proteins. They can be applied for tracking and ultrahigh-resolution localization of the single markers. In addition the spin properties of diamond defects can be utilized for novel magneto-optical imaging (MOI) with nanometer resolution. We develop this technique to unravel the details of the rotary motions and the elastic energy storage mechanism of a single biological nanomotor FoF1-ATP synthase. FoF1-ATP synthase is the enzyme that provides the 'chemical energy currency' adenosine triphosphate, ATP, for living cells. The formation of ATP is accomplished by a stepwise internal rotation of subunits within the enzyme. Previously subunit rotation has been monitored by single-molecule fluorescence resonance energy transfer (FRET) and was limited by the photostability of the fluorophores. Fluorescent nanodiamonds advance these FRET measurements to long time scales.

Affiliated with

Also associated with

No associations

Rating

Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-296420