Electrophoretic separation of large DNAs using steric confinement

Details Electrophoretic separation of large DNAs using steric confinement Electrophoretic separation of large DNAs using steric confinement

2006-10-12

arxiv.org/abs/cond-mat/0610328v2

Physics

Condensed Matter

Soft Condensed Matter

to appear in JCIS

Scientific paper

We report an alternative method for electrophoretic separation of large DNAs using steric confinement between solid walls, without gel or obstacles. The change of electrophoretic mobility vs confinement thickness is investigated using fluorescence video microscopy. We observe separation at small confinement thicknesses followed by a transition to the bulk behaviour (no separation) at a thickness of about 4 μm (a few radii of gyration for the studied DNA chains). We present tentative explanations of our original observations.

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