Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

Details Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

2007-10-05

arxiv.org/abs/0710.1240v2

Colloids Surf B 64 (2008) 77-87

Physics

Condensed Matter

Soft Condensed Matter

13 pages, 10 figures, accepted for publication in Colloids and Surfaces B: Biointerfaces

Scientific paper

10.1016/j.colsurfb.2008.01.004

A recently described technique (Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152--160) for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined. By observing a series of osmotic swellings followed by vesicle bursts upon a micropipette transfer of a single POPC GUV from a sucrose solution into an isoosmolar glycerol solution, a value for the permeability of POPC membrane for glycerol, P = (2.09+/-0.82) x 10^{-8} m/s, has been obtained. Based on this result, an alternative mechanism is proposed for the observed exchange of vesicle interior. With modifications, the method of Estes and Mayer is then applied to preparation of flaccid GUVs.

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