Efficiency of DNA replication in the Polymerase Chain Reactio

Details Efficiency of DNA replication in the Polymerase Chain Reactio Efficiency of DNA replication in the Polymerase Chain Reactio

2001-05-04

arxiv.org/abs/cond-mat/0105099v1

Proceedings of the National Academy of Sciences Volume 93, Number 23; Pages 12947-12952, November 12, 1996

Physics

Condensed Matter

Statistical Mechanics

4 figures

Scientific paper

We present a detailed kinetic model for the Polymerase Chain Reaction, and model the probability of replication in terms of the physical parameters of the problem. Applying the theory of branching processes, we show the existance of a new phenomenon affecting the probability distribution function of the number of replicants: for small number of initial molecules, the limiting behavior (with increasing number of cycles) of the pdf is represented by a multi-modal funtion.

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