Biology – Quantitative Biology – Biomolecules
Scientific paper
2007-05-24
Biology
Quantitative Biology
Biomolecules
14 pages, 3 figures. To appear in the International Journal of Nanotechnology and Molecular Computation. Improved background,
Scientific paper
In this paper we describe a new technique for the comparison of populations of DNA strands. Comparison is vital to the study of ecological systems, at both the micro and macro scales. Existing methods make use of DNA sequencing and cloning, which can prove costly and time consuming, even with current sequencing techniques. Our overall objective is to address questions such as: (i) (Genome detection) Is a known genome sequence present, at least in part, in an environmental sample? (ii) (Sequence query) Is a specific fragment sequence present in a sample? (iii) (Similarity discovery) How similar in terms of sequence content are two unsequenced samples? We propose a method involving multiple filtering criteria that result in "pools" of DNA of high or very high purity. Because our method is similar in spirit to hashing in computer science, we call it DNA hash pooling. To illustrate this method, we describe protocols using pairs of restriction enzymes. The in silico empirical results we present reflect a sensitivity to experimental error. Our method will normally be performed as a filtering step prior to sequencing in order to reduce the amount of sequencing required (generally by a factor of 10 or more). Even as sequencing becomes cheaper, an order of magnitude remains important.
Amos Martyn
Shasha Dennis
No associations
LandOfFree
DNA Hash Pooling and its Applications does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with DNA Hash Pooling and its Applications, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA Hash Pooling and its Applications will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-606357