The self-assembly of DNA Holliday junctions studied with a minimal model

Details The self-assembly of DNA Holliday junctions studied with a minimal model The self-assembly of DNA Holliday junctions studied with a minimal model

2008-07-21

arxiv.org/abs/0807.3280v2

J. Chem. Phys. 130, 065101 (2009)

Physics

Condensed Matter

Soft Condensed Matter

13 pages, 14 figures

Scientific paper

10.1063/1.3055595

In this paper, we explore the feasibility of using coarse-grained models to simulate the self-assembly of DNA nanostructures. We introduce a simple model of DNA where each nucleotide is represented by two interaction sites corresponding to the phosphate-sugar backbone and the base. Using this model, we are able to simulate the self-assembly of both DNA duplexes and Holliday junctions from single-stranded DNA. We find that assembly is most successful in the temperature window below the melting temperatures of the target structure and above the melting temperature of misbonded aggregates. Furthermore, in the case of the Holliday junction, we show how a hierarchical assembly mechanism reduces the possibility of becoming trapped in misbonded configurations. The model is also able to reproduce the relative melting temperatures of different structures accurately, and allows strand displacement to occur.

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