Physics – Condensed Matter – Mesoscale and Nanoscale Physics
Scientific paper
2011-08-31
J. Am. Chem. Soc., 2011, 133 (21), pp 8090-8093
Physics
Condensed Matter
Mesoscale and Nanoscale Physics
Scientific paper
10.1021/ja200967z
Single molecule applications, saturated pattern excitation microscopy, or stimulated emission depletion (STED) microscopy demand for bright and highly stable fluorescent dyes1,2. Despite of intensive research the choice of fluorphores is still very limited. Typically a stable fluorescent dyes is covalently attached to the target. This methodology brings forward a number of limitations, in particular, in case of protein labeling. First of all the fluorescent probes need to be attached selectively and site-specifically to prevent unspecific background. This often requires single cysteine mutations for covalent protein modification. Employing quantum dots allows overcoming problems of photo-bleaching3-6. However, the downsides are their large size, rendering the probe inaccessible to spatially confined architectures, issues in biocompatibility due to proper particle coating, and cellular toxicity6-8. Here we propose a new method to overcome the above outlined problems.
Choquet Daniel
Cognet Laurent
Giannone Gregory
Grunwald Christian
Lounis Brahim
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