A Solid-State System for High Temporal Resolution Fluorescence Lifetime Measurements

Details A Solid-State System for High Temporal Resolution Fluorescence Lifetime Measurements A Solid-State System for High Temporal Resolution Fluorescence Lifetime Measurements

2008-03-20

arxiv.org/abs/0803.3086v1

Physics

Condensed Matter

Soft Condensed Matter

9 pages, 4 figures

Scientific paper

A system for time-correlated single photon counting applications based on CMOS single photon avalanche diodes is presented. An instrument response function of 79 ps allows a fluorescence decay of high-affinity Ca2+ indicator Oregon Green BAPTA-1 to be analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay model is shown to best fit the fluorescence dynamics of OGB-1.

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