How to Track Protists in Three Dimensions

Details How to Track Protists in Three Dimensions How to Track Protists in Three Dimensions

2009-01-10

arxiv.org/abs/0901.1377v1

Rev. Sci. Instrum. 80, 014301 (2009)

Physics

Condensed Matter

Soft Condensed Matter

8 pages, 7 figures

Scientific paper

10.1063/1.3053242

We present an apparatus optimized for tracking swimming microorganisms in the size range 10-1000 microns, in three dimensions (3D), far from surfaces, and with negligible background convective fluid motion. CCD cameras attached to two long working distance microscopes synchronously image the sample from two perpendicular directions, with narrowband dark-field or bright-field illumination chosen to avoid triggering a phototactic response. The images from the two cameras can be combined to yield 3D tracks of the organism. Using additional, highly directional broad-spectrum illumination with millisecond timing control the phototactic trajectories in 3D of organisms ranging from Chlamydomonas to Volvox can be studied in detail. Surface-mediated hydrodynamic interactions can also be investigated without convective interference. Minimal modifications to the apparatus allow for studies of chemotaxis and other taxes.

Affiliated with

Also associated with

No associations

Rating

How to Track Protists in Three Dimensions does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with How to Track Protists in Three Dimensions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and How to Track Protists in Three Dimensions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-441222