Physics – Condensed Matter – Statistical Mechanics
Scientific paper
2002-01-21
Physics
Condensed Matter
Statistical Mechanics
12 pages, 3 figures, to appear in Biophysical Journal (April 2002) Due to an unfortunate name mismatch, the original submissio
Scientific paper
10.1016/S0006-3495(02)75563-X
In this work, an improved methodology for studying interactions of proteins in solution by small-angle scattering, is presented. Unlike the most common approach, where the protein-protein correlation functions $g_{ij}(r)$ are approximated by their zero-density limit (i.e. the Boltzmann factor), we propose a more accurate representation of $g_{ij}(r)$ which takes into account terms up to the first order in the density expansion of the mean-force potential. This improvement is expected to be particulary effective in the case of strong protein-protein interactions at intermediate concentrations. The method is applied to analyse small angle X-ray scattering data obtained as a function of the ionic strength (from 7 to 507 mM) from acidic solutions of $\beta $-Lactoglobuline at the fixed concentration of 10 $\rm g L^{-1}$. The results are compared with those obtained using the zero-density approximation and show a significant improvement particularly in the more demanding case of low ionic strength.
Carsughi Flavio
Gazzillo Domenico
Giacometti Achille
Mariani Paolo
Spinozzi Francesco
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