DNA ``comet assay'' for rapid detection of irradiated food

Details DNA ``comet assay'' for rapid detection of irradiated food DNA ``comet assay'' for rapid detection of irradiated food

Sep 1996

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1996rapc...48..381d&link_type=abstract

Radiation Physics and Chemistry, Volume 48, Issue 3, p. 381-381.

Physics

Scientific paper

Since treatment with ionizing radiation causes DNA fragmentation, microgel electrophoresis of single cell (``comet assay'') can be applied as a simple and rapid tool for identification of irradiated foods. The DNA ``comet assay'' has been employed successfully in the past to frozen meats (chicken, pork, beef) and its application is now being extended to a variety of other food items, such as fish, fruits, legumes, seeds and even spices. The electrophoretic separation requires only a few minutes, and after visualising DNA by silver staining, the DNA fragmentation pattern observed in a simple transmission microscope may indicate a possible irradiation treatment. Suspected samples may subsequently be analyzed by the more sophisticated (expensive) and validated techniques.

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