Physics
Scientific paper
Sep 1988
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1988gecoa..52.2257s&link_type=abstract
Geochimica et Cosmochimica Acta, vol. 52, Issue 9, pp.2257-2267
Physics
1
Scientific paper
Humic acids are the predominant source of error in the 14 C and stable isotope analysis of fossil bone organic matter. XAD-2 resin will quantitatively remove humates and give the highest yields of protein from bones with variable types of preservation. Decalcified bone, gelatin and base-leached residues can vary up to 5%. for 13 C and by 1%. on 15 N relative to XAD-treated fractions. Simultaneous analysis of 14 C age, 13 C and 15 N is recommended because each isotope value can be independently affected by the bone's diagenetic history. Radiocarbon analysis is the most sensitive and 15 N is least sensitive for detecting exogenous organic matter. The uncertainty of analyses on the best pretreated protein is ±0.5%. for both 13 C and 15 N and is larger than previous estimates. The accuracy for all isotope analyses will be better assessed by using individual amino acids instead of total collagenous residues. Inaccurate 14 C dates on severely degraded bone are an indication that this class of fossils may be unsuitable for any isotopic analysis.
Brendel Klaus
Duhamel Raymond C.
Stafford Thomas W. Jr.
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