Influence of the pinhole size on the resolution of the 4Pi' microscope studied by means of the optical transfer function

Physics – Optics

Scientific paper

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Scientific paper

10.1016/j.nima.2006.10.120

Optical transfer functions are presented for the 4pi' microscope. The effects
of the size of the confocal pinhole on the three-dimensional resolution are
studied. It is shown that the resolution of the 4Pi' microscope depends weakly
on the pinhole size. This result suggest that the pinhole can be removed in
order to increase the signal-to-noise ratio.

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