Effect of intense, ultrashort laser pulses on DNA plasmids in their native state: strand breakages induced by {\it in-situ} electrons and radicals

Details Effect of intense, ultrashort laser pulses on DNA plasmids in their native state: strand breakages induced by {\it in-situ} electrons and radicals Effect of intense, ultrashort laser pulses on DNA plasmids in their native state: strand breakages induced by {\it in-situ} electrons and radicals

2011-02-15

arxiv.org/abs/1102.2995v1

Phys. Rev. Letters 106 (2011) 118101

Physics

Biological Physics

To appear in Phys. Rev. Letters

Scientific paper

Single strand breaks are induced in DNA plasmids, pBR322 and pUC19, in aqueous media exposed to strong fields generated using ultrashort laser pulses (820 nm wavelength, 45 fs pulse duration, 1 kHz repetition rate) at intensities of 1-12 TW cm$^{-2}$. The strong fields generate, {\it in situ}, electrons and radicals that induce transformation of supercoiled DNA into relaxed DNA, the extent of which is quantified. Introduction of electron and radical scavengers inhibits DNA damage; results indicate that OH radicals are the primary (but not sole) cause of DNA damage.

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