Cell-Free, De Nova Synthesis of Poliovirus

Details Cell-Free, De Nova Synthesis of Poliovirus Cell-Free, De Nova Synthesis of Poliovirus

Dec 1991

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1991sci...254.1647m&link_type=abstract

Science, Volume 254, Issue 5038, pp. 1647-1651

Physics

5

Scientific paper

Cell-free translation of poliovirus RNA in an extract of uninfected human (HeLa) cells yielded viral proteins through proteolysis of the polyprotein. In the extract, newly synthesized proteins catalyzed poliovirus-specific RNA synthesis, and formed infectious poliovirus de novo. Newly formed virions were neutralized by type-specific antiserum, and infection of human cells with them was prevented by poliovirus receptor-specific antibodies. Poliovirus synthesis was increased nearly 70-fold when nucleoside triphosphates were added, but it was abolished in the presence of inhibitors of translation or viral genome replication. The ability to conduct cell-free synthesis of poliovirus will aid in the study of picornavirus proliferation and in the search for the control of picornaviral disease.

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