Nonenzymatic Template-Directed Condensation of Short-Chained Oligouridylates on a Poly(A) Template

Details Nonenzymatic Template-Directed Condensation of Short-Chained Oligouridylates on a Poly(A) Template Nonenzymatic Template-Directed Condensation of Short-Chained Oligouridylates on a Poly(A) Template

Dec 2000

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2000oleb...30..503s&link_type=abstract

Origins of Life and Evolution of the Biosphere, v. 30, Issue 6, p. 503-511 (2000).

Physics

1

Nonenzymatic Template-Directedcondensation, Oligouridylates, Poly(A)

Scientific paper

An oligouridylate with chain-length of more than three was condensed on a polyadenylate [poly(A)] template with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) in imidazole buffer. The condensation reaction proceeds via the phosphorimidazolide of the oligouridylate as an intermediate. Pentauridylate [(pU)_5] was converted to decauridylate [(pU)_10] in 10% yield at 0 °C for 7 days in the presence of the poly(A) template, while no coupling product was obtained in the absence of the poly(A) template. The resulting linkage of the (pU)_10 was mainly 2'-5' linkage.

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