Vibrational imaging based on stimulated Raman scattering microscopy

Details Vibrational imaging based on stimulated Raman scattering microscopy Vibrational imaging based on stimulated Raman scattering microscopy

Mar 2009

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2009njph...11c3026n&link_type=abstract

New Journal of Physics, Volume 11, Issue 3, pp. 033026 (2009).

Physics

18

Scientific paper

A stimulated Raman scattering microscope with near-infrared picosecond laser pulses at high repetition rates (76 MHz) and radio-frequency lock-in detection is accomplished. Based on stimulated Raman loss detection, we demonstrate noninvasive point-by-point vibrational mapping of chemical and biological samples with high sensitivity and without the requirement for labeling of the sample with natural or artificial fluorophores. We experimentally demonstrate a major benefit of this technique, which is the capability to respond exclusively to the linear Raman-resonance properties of the sample, thus allowing a direct quantitative interpretation of image contrast in terms of the number density of Raman-active modes.

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