UVA-induced oxidative stress in single cells probed by autofluorescence modifications, cloning assay, and comet assay

Details UVA-induced oxidative stress in single cells probed by autofluorescence modifications, cloning assay, and comet assay UVA-induced oxidative stress in single cells probed by autofluorescence modifications, cloning assay, and comet assay

Jan 1996

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1996spie.2628...43k&link_type=abstract

Proc. SPIE Vol. 2628, p. 43-50, Optical and Imaging Techniques for Biomonitoring, Hans-Jochen Foth; Renato Marchesini; Halina Po

Physics

Scientific paper

Cell damage by low-power 365 nm radiation of a 50 W high-pressure mercury microscopy lamp was studied. UVA exposure to CHO cells resulted for radiant exposures greater than 10 kJ/m2 in significant modifications of NADH-attributed autofluorescence and in inhibition of cell division. Single cell gel electrophoresis (comet assay) revealed UVA-induced single strand DNA breaks. According to these results, UVA excitation radiation in fluorescence microscopy may damage cells. This has to be considered in vital cell microscopy, e.g. in calcium measurements.

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