Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

Details Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

Jan 1999

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1999jcp....96..143p&link_type=abstract

Journal de Chimie Physique et de Physico-Chimie Biologique, Volume 96, Issue 1, January 1999, pp.143-146

Physics

Γ Irradiation, Dna Damages, Photosensitization, Comet Assay

Scientific paper

We used the comet assay associated with DNA-glycosylases to estimate DNA damage in cells exposed to gamma irradiation or photosensitized either with methylene blue or orange acridine. A calibration performed using irradiation allowed the measurement of the steady-state level and the yield of 8-oxodGuo as well as strand breaks and alkali-labile sites.
Nous avons utilisé la méthode des comètes associée à des ADN-glycosylases, pour estimer les dommages de l'ADN dans des cellules après l'exposition à un rayonnement gamma ou après photosensibilisation par le bleu de méthylène ou l'acridine orange. Une calibration de la méthode des comètes a permis de mesurer le niveau basal et les taux de formation de 8-oxodGuo ainsi que le nombre de cassures de brins et de sites alcali labiles.

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