Ultrafast laser induced condensation of molecules

Details Ultrafast laser induced condensation of molecules Ultrafast laser induced condensation of molecules

Feb 2011

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2011spie.7925e...2g&link_type=abstract

Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI. Edited by Heisterkamp, Alexander; Neev,

Physics

Optics

Scientific paper

We have found that ultrafast laser microbeam inducing bubble(s) can lead to condensation of molecules in solution. The laser microbeam was generated by coupling a near infrared mode-locked femtosecond (fs) laser beam onto an inverted fluorescence microscope and focusing it with a 100X objective. Fluorescence imaging revealed that collapse of cavitation micro-bubbles created high-concentration regions of dye molecules in the aqueous solution. Further, twophoton excitation of the molecules under the ultrafast laser microbeam showed significant increase in fluorescence intensity as a function of laser exposure time without micro-bubble formation. This may be attributed to nano-bubble formation and or conformational change in the molecules under intense laser intensity at the focused spot to significantly enhance the molar absorptivity (extinction coefficient) or fluorescence excitation cross-section. High-concentration regions of the dye molecules are found to be retained for a longer period of time and therefore provide an opportunity for collection of these condensed molecules using microcapilary and/or for further analysis.

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