The use of reduced temperatures for reversible developmental arrest of organ cultures prior to spaceflight experimentation and for postflight analyses

Details The use of reduced temperatures for reversible developmental arrest of organ cultures prior to spaceflight experimentation and for postflight analyses The use of reduced temperatures for reversible developmental arrest of organ cultures prior to spaceflight experimentation and for postflight analyses

Jan 1997

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1997aipc..387..933k&link_type=abstract

Space technology and applications international forum (STAIF - 97). AIP Conference Proceedings, Volume 387, pp. 933-938 (1997).

Physics

Medical Physics

Aerospace Bio- And Medical Physics

Scientific paper

One complication of using rapidly growing and developing tissues for spaceflight experimentation is that, due to early turnover and launch delays, the tissues often undergo complete development before orbit is achieved. We conducted a series of studies using three different types of tissue, chick pre-cardiac explants, embryonic mouse lung rudiments and embryonic mouse pre-metatarsal mesenchyme, to examine the use of reduced temperature as an inexpensive means to slow growth and development, before the experiment begins. Pre-cardiac explants could be held at 4 °C (277K), 13 °C (286K), or 22 °C (295K) for up to 48 hours and still begin normal beating within 24 hours of culture at 37 °C (310K). Lung explants could be held at 5 °C (278K), 15 °C (288K), and 24 °C (297K) for 3-6 days without clefts changing in appearance, but would resume branching morphogenesis and growth after being placed at 37 °C (310K). Pre-metatarsal cultures could be held at 15 °C (288K), 22 °C (295K) and 24 °C (297K) for 6 days with very little change in rod length. After additional incubation at 37 °C (310K) the rods increased in length and mineralized. These results suggest that incubation at temperatures below standard culture temperature are capable of slowing tissue growth, but growth and development will resume after standard incubation.

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