The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites

Physics – Biological Physics

Scientific paper

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Scientific paper

We present investigations on volume regulation and beading shape transitions in PC12 neurites conducted using a flow-chamber technique. By disrupting the cell cytoskeleton with specific drugs we investigate the role of its individual components in the volume regulation response. We find that microtubule disruption increases both swelling rate and maximum volume attained, but does not affect the ability of the neurite to recover its initial volume. In addition, investigation of axonal beading --also known as pearling instability-- provides additional clues on the mechanical state of the neurite. We conclude that the initial swelling phase is mechanically slowed down by microtubules, while the volume recovery is driven by passive diffusion of osmolites. Our experiments provide a framework to investigate the role of cytoskeletal mechanics in volume homeostasis.

No associations

LandOfFree

Say what you really think

Search LandOfFree.com for scientists and scientific papers. Rate them and share your experience with other people.

Rating

The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFWR-SCP-O-29949

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.