Physics
Scientific paper
Aug 2010
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2010njph...12h3037l&link_type=abstract
New Journal of Physics, Volume 12, Issue 8, pp. 083037 (2010).
Physics
2
Scientific paper
In this paper, we study the application of a plasma needle to induce necrosis in planktonic samples containing a single breed of bacteria. Two different types of bacteria, Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922), were covered in this study. In all experiments with bacteria, the samples were liquid suspensions of several different concentrations of bacteria prepared according to the McFarland standard. The second system studied in this paper was human peripheral blood mesenchymal stem cells (hPB-MSC). In the case of hPB-MSC, two sets of experiments were performed: when cells were covered with a certain amount of liquid (indirect) and when the cell sample was in direct contact with the plasma.
Most importantly, the study is made with the aim to see the effects when the living cells are in a liquid medium, which normally acts as protection against the many agents that may be released by plasmas. It was found that a good effect may be expected for a wide range of initial cell densities and operating conditions causing destruction of several orders of magnitude even under the protection of a liquid. It was established independently that a temperature increase could not affect the cells under the conditions of our experiment, so the effect could originate only from the active species produced by the plasma. In the case of those hPB-MSC that were not protected by a liquid, gas flow proved to produce a considerable effect, presumably due to poor adhesion of the cells, but in a liquid the effect was only due to the plasma. Further optimization of the operation may be attempted, opening up the possibility of localized in vivo sterilization.
Bugarski Diana
Jovanović Milena
Lazovic Saša
Maletic Dejan
Malović Gordana
No associations
LandOfFree
The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-1123104