Surfaces and orientations: much to fret about?

Details Surfaces and orientations: much to fret about? Surfaces and orientations: much to fret about?

2004-12-07

arxiv.org/abs/q-bio/0412013v1

Biology

Quantitative Biology

Biomolecules

9 pages, 7 figures

Scientific paper

Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this review, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.

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