Biology – Quantitative Biology – Biomolecules
Scientific paper
2005-03-07
Chinese Science Bulletin, Vol.50, No. 8 (2005) 731-737
Biology
Quantitative Biology
Biomolecules
11 pages, 7 figures
Scientific paper
The effects of monovalent (Na+, K+) and divalent (Mg2+, Ca2+, Mn2+) ions on the interaction between DNA and histone are studied using the molecular combing technique. Lamda-DNA molecules and DNA-histone complexes incubated with metal cations (Na+, K+, Mg2+, Ca2+, Mn2+) are stretched on hydrophobic surfaces, and directly observed by fluorescence microscopy. The results indicate that when these cations are added into the DNA solution, the fluorescence intensities of the stained DNA are reduced differently. The monovalent cations (Na+, K+) inhibit binding of histone to DNA. The divalent cations (Mg2+, Ca2+, Mn2+) enhance significantly the binding of histone to DNA and the binding of the DNA-histone complex to the hydrophobic surface. Mn2+ also induces condensation and aggregation of the DNA-histone complex.
Dou Shuo-Xing
Liu Yu-Ying
Wang Peng-Ye
Wang Wei-Chi
Xie Ping
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