Physics – Biological Physics
Scientific paper
2011-10-06
Appl. Phys. Lett. 99, 133701 (2011)
Physics
Biological Physics
Scientific paper
10.1063/1.3645010
For single particle electron cryo-microscopy (cryoEM), contrast loss due to beam-induced charging and specimen movement is a serious problem, as the thin films of vitreous ice spanning the holes of a holey carbon film are particularly susceptible to beam-induced movement. We demonstrate that the problem is at least partially solved by carbon nanotechnology. Doping ice-embedded samples with single-walled carbon nanotubes (SWNT) in aqueous suspension or adding nanocrystalline graphene supports, obtained by thermal conversion of cross-linked self-assembled biphenyl precursors, significantly reduces contrast loss in high-resolution cryoEM due to the excellent electrical and mechanical properties of SWNTs and graphene.
Gölzhäuser Armin
Kühlbrandt Werner
Rhinow Daniel
Turchanin Andrey
Weber Nils-Eike
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