Salt RNA protection against thermodegradation

Details Salt RNA protection against thermodegradation Salt RNA protection against thermodegradation

Apr 2003

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2003eaeja.....2718v&link_type=abstract

EGS - AGU - EUG Joint Assembly, Abstracts from the meeting held in Nice, France, 6 - 11 April 2003, abstract #2718

Physics

Scientific paper

We shown the structural integrity of tRNA at high temperature, 82^oC for 30h, in high salt concentrations (Tehei et al, 2002). Stability were also performed by measuring the residual specific tRNA charge capacity after heat treatment for 30 h at 82^oC. We have undertaken in vitro selection of RNA molecules at high temperature in presence of an ancient halite (NaCl) sample (reference : EZ08-K6-C9). This sample, collected in a borehole at 720.15 m depth, belongs to the Rupelian Upper Salt Formation of the Bresse salt basin (France). Its age is estimated to about 31±3 millions years. These studies provide support for the importance of salt to protect macromolecules against thermal degradation allowing activity to be recovered. These could be useful for searching traces of life in ancient sediments and in planetary exploration. Reference: Tehei Moeva, Franzetti Bruno, Maurel Marie-Christine, Vergne Jacques, Hountondji Codjo and Zaccai Giuseppe, Extremophiles, (2002), 6: 427-430.

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