Physics – Biological Physics
Scientific paper
2010-12-03
Physics
Biological Physics
Scientific paper
RSC is an essential chromatin remodeling factor that is required for the control of several processes including transcription, repair and replication. The ability of RSC to relocate centrally positioned mononucleosomes at the end of nucleosomal DNA is firmly established, but the data on RSC action on oligo-nucleosomal templates remains still scarce. By using Atomic Force Microscopy (AFM) imaging, we have quantitatively studied the RSC- induced mobilization of positioned di- and trinucleosomes as well as the directionality of mobilization on mononucleosomal template labeled at one end with streptavidin. AFM imaging showed only a limited set of distinct configurational states for the remodeling products. No stepwise or preferred directionality of the nucleosome motion was observed. Analysis of the corresponding reaction pathways allows deciphering the mechanistic features of RSC-induced nucleosome relocation. The final outcome of RSC remodeling of oligosome templates is the packing of the nucleosomes at the edge of the template, providing large stretches of DNA depleted of nucleosomes. This feature of RSC may be used by the cell to overcome the barrier imposed by the presence of nucleosomes.
Angelov Dimitar
Castelnovo Martin
Dimitrov Stefan
Faivre-Moskalenko Cendrine
Menoni Hervé
No associations
LandOfFree
RSC remodeling of oligo-nucleosomes: an atomic force microscopy study does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with RSC remodeling of oligo-nucleosomes: an atomic force microscopy study, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and RSC remodeling of oligo-nucleosomes: an atomic force microscopy study will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-515180