Resonance Raman measurements of carotenoids using light emitting diodes

Details Resonance Raman measurements of carotenoids using light emitting diodes Resonance Raman measurements of carotenoids using light emitting diodes

2008-01-10

arxiv.org/abs/0801.1697v2

Physics

Medical Physics

Accepted for publication by the Journal of Biomedical Optics

Scientific paper

10.1117/1.2952075

We report on the development of a compact commercial instrument for measuring carotenoids in skin tissue. The instrument uses two light emitting diodes (LEDs) for dual-wavelength excitation and four photomultiplier tubes for multichannel detection. Bandpass filters are used to select the excitation and detection wavelengths. The f/1.3 optical system has high optical throughput and single photon sensitivity, both of which are crucial in LED-based Raman measurements. We employ a signal processing technique that compensates for detector drift and error. The sensitivity and reproducibility of the LED Raman instrument compares favorably to laser-based Raman spectrometers. This compact, portable instrument is used for non-invasive measurement of carotenoid molecules in human skin with a repeatability better than 10%.

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