Resolution and enhancement in nanoantenna-based fluorescence microscopy

Details Resolution and enhancement in nanoantenna-based fluorescence microscopy Resolution and enhancement in nanoantenna-based fluorescence microscopy

2009-10-29

arxiv.org/abs/0910.5593v1

Physics

Optics

10 pages, 3 figures. accepted for publication in Nano Letters

Scientific paper

Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near-fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the diameter of the gold nanoparticle. We examine the interplay between fluorescence enhancement and spatial resolution and discuss the requirements for deciphering single molecules in a dense sample. Resolutions better than 20 nm and fluorescence enhancement up to 30 times are demonstrated experimentally. By accounting for the tip shaft and the sample interface in finite-difference time-domain calculations, we explain why the measured fluorescence enhancements are higher in the presence of an interface than the values predicted for a homogeneous environment.

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