Reconstructing an Icosahedral Virus from Single-Particle Diffraction Experiments

Details Reconstructing an Icosahedral Virus from Single-Particle Diffraction Experiments Reconstructing an Icosahedral Virus from Single-Particle Diffraction Experiments

2011-07-26

arxiv.org/abs/1107.5212v1

Biology

Quantitative Biology

Biomolecules

18 pages, 10 figures

Scientific paper

The first experimental data from single-particle scattering experiments from free electron lasers (FELs) are now becoming available. The first such experiments are being performed on relatively large objects such as viruses, which produce relatively low-resolution, low-noise diffraction patterns in so-called "diffract-and-destroy" experiments. We describe a very simple test on the angular correlations of measured diffraction data to determine if the scattering is from an icosahedral particle. If this is confirmed, the efficient algorithm proposed can then combine diffraction data from multiple shots of particles in random unknown orientations to generate a full 3D image of the icosahedral particle. We demonstrate this with a simulation for the satellite tobacco necrosis virus (STNV), the atomic coordinates of whose asymmetric unit is given in Protein Data Bank entry 2BUK.

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