Physics
Scientific paper
Mar 1971
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1971natur.230...52b&link_type=abstract
Nature, Volume 230, Issue 5288, pp. 52 (1971).
Physics
2
Scientific paper
RECENT observations1-4 of specific localized fluorescence in Y chromosomes stained with quinacrine mustard and quinacrine hydrochloride have provided a new parameter for the study of abnormal Y chromosomes. We have re-examined regularly prepared chromosome and buccal smear slides of at least 100 cells from two patients with a small Y chromosome5 and from patients with normal Y chromosomes. We used a modification of techniques described earlier1-4. We stained for 5 min in 0.5% quinacrine hydrochloride (Winthrop); washed in distilled water; differentiated for 3 min in citric acid-phosphate buffer, pH 5.5; washed and mounted slides in phosphate buffer, pH 7.4, and sealed with nail polish. Preparations were examined with a Leitz Ortholux microscope with a mercury arc lamp, standard fluorescence filters, and dark-field illumination, and were photographed with Ektachrome high speed film. Fluorescence persisted for several days. Full details of the technique will be published later.
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