Quantitative Determination of Spatial Protein-protein Proximity in Fluorescence Confocal Microscopy

Details Quantitative Determination of Spatial Protein-protein Proximity in Fluorescence Confocal Microscopy Quantitative Determination of Spatial Protein-protein Proximity in Fluorescence Confocal Microscopy

2009-03-05

arxiv.org/abs/0903.1121v2

Physics

Biological Physics

19 pages, 5 figures

Scientific paper

10.1016/j.bpj.2008.12.052

To quantify spatial protein-protein proximity (colocalization) in fluorescence microscopic images, cross-correlation and autocorrelation functions were decomposed into fast and slowly decaying components. The fast component results from clusters of proteins specifically labeled and the slow one from background/image heterogeneity. We show that the calculation of the protein-protein proximity index and the correlation coefficient are more reliably determined by extracting the fast-decaying component.

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