Physics – Optics
Scientific paper
Feb 2011
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2011spie.7931e..16j&link_type=abstract
MEMS Adaptive Optics V. Edited by Olivier, Scot S.; Bifano, Thomas G.; Kubby, Joel A. Proceedings of the SPIE, Volume 7931, p
Physics
Optics
Scientific paper
Inhomogeneous optical properties of biological samples make it difficult to obtain diffraction-limited resolution in depth. Correcting the sample-induced optical aberrations needs adaptive optics (AO). However, the direct wavefront-sensing approach commonly used in astronomy is not suitable for most biological samples due to their strong scattering of light. We developed an image-based AO approach that is insensitive to sample scattering. By comparing images of the sample taken with different segments of the pupil illuminated, local tilt in the wavefront is measured from image shift. The aberrated wavefront is then obtained either by measuring the local phase directly using interference or with phase reconstruction algorithms similar to those used in astronomical AO. We implemented this pupil-segmentation-based approach in a two-photon fluorescence microscope and demonstrated that diffraction-limited resolution can be recovered from nonbiological and biological samples.
Betzig Eric
Ji Na
Milkie Daniel E.
No associations
LandOfFree
Pupil-segmentation-based adaptive optics for microscopy does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Pupil-segmentation-based adaptive optics for microscopy, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Pupil-segmentation-based adaptive optics for microscopy will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-1534068