Physics – Medical Physics
Scientific paper
Jan 1997
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1997aipc..387.1019t&link_type=abstract
Space technology and applications international forum (STAIF - 97). AIP Conference Proceedings, Volume 387, pp. 1019-1024 (1997
Physics
Medical Physics
Aerospace Bio- And Medical Physics
Scientific paper
The method of crop improvement by gene transfer is becoming increasingly routine with transgenic foods and ornamental crops now being marketed to consumers. However, biological processes of plants, and the physical barriers of current protocols continue to limit the application of gene transfer in many commercial crops. The goal of this paper is to outline the current limitations of gene transfer and to hypothesize possible opportunities for use of microgravity to overcome such limitations. The limitations detailed in this paper include host-range specificity of Agrobacterium mediated transformation, probability of gene insertion, position effects of the inserted genes, gene copy number, stability of foreign gene expression in host plants, and regeneration of recalcitrant plant species. Microgravity offers an opportunity for gene transfer where cell growth kinetics, DNA synthesis, and genetic recombination rates can be altered. Such biological conditions may enhance the ability for recombination of reporter genes and other genes of interest to agriculture. Proposed studies would be useful for understanding instability of foreign gene expression and may lead to stable transformed plants. Other aspects of gene transfer in microgravity are discussed.
Tennessen Daniel J.
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