Probing embryonic tissue mechanics with laser hole-drilling

Physics – Biological Physics

Scientific paper

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

23 pages with 9 figures (require color)

Scientific paper

10.1088/1478-3975/6/3/036004

We use laser hole-drilling to assess the mechanics of an embryonic epithelium during development - in vivo and with subcellular resolution. We ablate a subcellular cylindrical hole clean through the epithelium, and track the subsequent recoil of adjacent cells (on ms time scales). We investigate dorsal closure in the fruit fly with emphasis on apical constriction of amnioserosa cells. The mechanical behavior of this epithelium falls between that of a continuous sheet and a 2D cellular foam (a network of tensile interfaces). Tensile stress is carried both by cell-cell interfaces and by the cells' apical actin networks. Our results show that stress is slightly concentrated along interfaces (1.6-fold), but only in early closure. Furthermore, closure is marked by a decrease in the recoil power-law exponent - implying a transition to a more solid-like tissue. We use the site- and stage-dependence of the recoil kinetics to constrain how the cellular mechanics change during closure. We apply these results to test extant computational models.

No associations

LandOfFree

Say what you really think

Search LandOfFree.com for scientists and scientific papers. Rate them and share your experience with other people.

Rating

Probing embryonic tissue mechanics with laser hole-drilling does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Probing embryonic tissue mechanics with laser hole-drilling, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Probing embryonic tissue mechanics with laser hole-drilling will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFWR-SCP-O-668846

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.